In the liver, acyl-CoA, generated from free fatty acids, is transferred into the mitochondria via the carnitine shuttle and is converted to acetyl-CoA, which is metabolized in the TCA cycle
We modeled the aging effects using the following data: expression fold changes of enzymes and transporters from the Tabula Muris Senis (TMS) dataset ( scaled initial concentrations of blood glucose, lactate, and -hydroxybutyrate according to the literature data on difference in their levels in aging (approximation, because effect size depends on the literature source) total NAD + and NADH concentration pool scaling (approximation), because it decreases in aging according to qualitative literature synaptic glutamate release pool (approximation, but synaptic input is set as the same for comparability of the results) scaling of reducing equivalents shuttles between cytosol and mitochondria: the NADH shuttle is a generalized rate equation based on the activity of multiple enzymes of malate-aspartate and glycerol-phosphate shuttles, for which we followed the literature to model it ( For the above factors, which mention approximative/approximation, the direction of change is according to the literature, but the absolute number of scaling factors (not known/contradictory in the literature) is set with an objective for the model to be steady at rest
Sherer TB, Betarbet R, Stout AK, Lund S, Baptista M, Panov AV, et al
Circadian disruption is now linked to insulin resistance, elevated cortisol, elevated inflammatory markers, and accelerated biological aging
Glutathione peroxidase is an enzyme, specifically a family of enzymes, involved in the breakdown of hydrogen peroxide and lipid peroxides
